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KU Home  :  Pharmaceutical Chemistry  :  Pharmaceutical Chemistry - Faculty - John F. Stobaugh Ph.D.

John F. Stobaugh Ph.D.
Professor, Courtesy Professor of Chemistry Associate Dean of Research & Graduate Affairs
Departments: Pharmaceutical Chemistry
Office: 136A Simons
Email: Stobaugh@ku.edu
Phone: (785) 864-3996
Fax: (785) 864-5736

Educational Background:

B.S., 1974, The University of Oklahoma
M.S., 1977, The University of Oklahoma
Ph.D. 1982, The University of Kansas

Research Group:

  • Leon van Haandel: Graduate Student
  • Josh Woods: Graduate Student

Research Interests:

Capillary electrochromatography, capillary electrophoresis, chiral separations, bio-macromolecule separations, analytical derivatization reagents, bioanalytical chemistry, pharmaceutical analysis, anticancer agents

Professor Stobaugh's research emphasizes liquid phase capillary column separation techniques (capillary electrochromatography, capillary liquid chromatography and capillary electrophoresis) and various chemistries for the enhancement of separation and detection. Application of these technologies is directed towards the in vivo analysis of anticancer agents, drugs, and peptides and rapid high efficiency separations of bio-macromolecules. Active projects include the development of fluorogenic reagents that enable laser-induced fluorescence (LIF) detection of amino acids and peptides and electrogenic derivatization reagents; the development of novel chiral selectors for the resolution of stereoisomers when used in conjunction with capillary electrophoresis (CE); and the utilization and development of capillary electrochromatography (CEC) as a routine technique for pharmaceutical and biomedical analysis.

A recent result in the bioanalysis of an anticancer agent is shown as an example. In the figure, the fluorogenic derivatization of DFMO is accomplished using the reagent Imidazole-NDA. The fluorescent product is then separated from other substances in blood by CE with detection being accomplished using an Agron Ion-based LIF detector.

In a second example, packed column CEC is used to rapidly resolve a mixture of pharmaceutical substances that are closely related. The very high efficiency allows for the rapid separation time.

Overall, the research programs in these laboratories seek to develop advanced approaches for the acquisition, preparation, separation and quantitation of analytes of pharmaceutical and biomedical importance.

Current Research Projects:

 
 

 : Stobaugh Lab Group

Tyrosine oxidation has been linked to various age dependent pathologies including atherosclerosis and cataracts

A novel stable isotope derivatization strategy was developed for mass spectrometry based relative quantitation

Analytical techniques included LC-MS, Fluorescence Spectroscopy, NMR, X-ray analysis
 
 
 
 

 : Stobaugh Lab Group

Monolithic silica columns where fabricated for use in separating 5 basic amine containing drugs

Monolithic columns show increased efficiency and lessen the difficulties associated with high linear velocities

Additionally, the pure nature of the silica surface allows for an ideal column for use in HILIC chromatographic analysis
 
 

Publications:

M. J. Rose, S. M. Lunte, R. G. Carlson and J. F. Stobaugh, "Amino acid and peptide analysis using derivatization with p-nitrophenol-2,5-dihydroxyphenylacetate bis-tetrahydropyranyl ether and capillary electrophoresis with electrochemical detection," J. Pharm. Biomed. Anal., 30: 1851–1859 (2003).

J. Z. Yang, K. C. Bastian, R. D. Moore, J. F. Stobaugh and R. T. Borchardt, "Quantitative analysis of a model opioid peptide and its cyclic prodrugs in rat plasma using high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection," J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 780: 269–281 (2002).

S. E. Moring, O. S. Wong and J. F. Stobaugh, "Target specific sample preparation from aqueous extracts with molecular imprinted polymers," J. Pharm. Biomed. Anal., 27: 719–728 (2002).

C. Ampasavate, G. A. Chandorkar, D. G. Vande Velde, J. F. Stobaugh and K. L. Audus, "Transport and metabolism of opioid peptides across BeWo cells, an in vitro model of the placental barrier," Int. J. Pharm., 233: 85–98 (2002).

M. J. Rose, S. M. Lunte, R. G. Carlson and J. F. Stobaugh, "Transformation of analytes for electrochemical detection: a review of chemical and physical approaches," Adv. Chromatogr., 41: 203–248 (2001).

P. D. Angus, C. W. Demarest, T. Catalano and J. F. Stobaugh, "Aspects of column fabrication for packed capillary electrochromatography," J. Chromatogr. A, 887: 347–365 (2000).

D. J. Skanchy, G. H. Xie, R. J. Tait, E. Luna, C. Demarest and J. F. Stobaugh, "Application of sulfobutylether-beta-cyclodextrin with specific degrees of substitution for the enantioseparation of pharmaceutical mixtures by capillary electrophoresis," Electrophoresis, 20: 2638–2649 (1999).

M. J. Rose, S. M. Lunte, R. G. Carlson and J. F. Stobaugh, "Hydroquinone-based derivatization reagents for the quantitation of amines using electrochemical detection," Anal. Chem., 71: 2221–2230 (1999).

G. A. Chandorkar, C. Ampasavate, J. F. Stobaugh and K. L. Audus, "Peptide transport and metabolism across the placenta," Adv. Drug Deliv. Rev., 38: 59–67 (1999).

P. D. Angus, C. W. Demarest, T. Catalano and J. F. Stobaugh, "Evaluation of 1.5 microM reversed phase nonporous silica in packed capillary electrochromatography and application in pharmaceutical analysis," Electrophoresis, 20: 2349–2359 (1999).